Development and Validation of a Confirmatory Method for the Determination of 12 Coccidiostat Residues in Eggs and Muscle by Means of Liquid Chromatography Coupled to Hybrid High Resolution Mass Spectrometry

A confirmatory, highly selective multi-residue method based on liquid chromatography coupled to hybrid high resolution mass spectrometry (LC-Q-Orbitrap) was developed and validated for the determination of 12 regulated coccidiostats in eggs muscle. Particularly, ionophore antibiotics (lasalocid, maduramicin, monensin, narasin, salinomycin semduramicin) synthetic (diclazuril, halofuginone, nicarbazin as 4,4?-dinitrocarbanilide fraction, robenidine toltrazuril toltrazuril-sulphone) were included method. The sample preparation consisted extraction analytes from matrix with acetonitrile, followed by a clean-up step Oasis® PRiME HLB SPE defatting procedure n-hexane. Validation successfully performed according Commission Implementing Regulation (EU) 2021/808, starting 1 µg kg?1. verified through analysis certified reference material (CRM) occurrence residues assessed context Italian National Residue Control Plan (NRCP).